Clinical Labeling and Imaging of Transplanted CD133<sup>+</sup>/CD34<sup>+</sup> Stem Cells in Patients with Ischemic Heart Disease

Authors

  • Ali Ghodsizad
  • Viktor Bordel
  • Brian Bruckner
  • Mathias Loebe
  • Gunter Fuerst
  • I. Mirsaidi
  • M. Sucker
  • Arjang Ruhparwar
  • Matthias Karck
  • Hans Michael Klein

DOI:

https://doi.org/10.1532/HSF98.20111138

Abstract

The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133+ and CD34+ stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

References

Dick AJ, Guttman MA, Raman VK, et al. 2003. Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in swine. Circulation 108:2899-904.nGhodsizad A, Bara C Niehaus M, et al. 2006. In vivo echocardiographic imaging of transplanted human adult stem cells in the myocardium labeled with clinically applicable CliniMACS nanoparticles. J Am Soc Echocardiogr 19:563-8.nGhodsizad A, Klein HM, Borowski A, et al. 2004. Intraoperative isolation and processing of BM-derived stem cells. Cytotherapy 6:523-6.nKraitchman DL, Heldman AW, Atalar E, et al. 2003. In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation 107:2290-3.nOrlic D, Kajstura J, Chimenti S, et al. 2001. Bone marrow cells regenerate infarcted myocardium. Nature 410:701-5.nPesce M, Orlandi A, Iachininoto MG, et al. 2003. Myoendothelial differentiation of human umbilical cord blood-derived stem cells in ischemic limb tissues. Circ Res 93:e51-62.nQuirici N, Soligo D, Caneva L, Servida F, Bossolasco P, Deliliers GL. 2001. Differentiation and expansion of endothelial cells from human bone marrow CD133+ cells. Br J Haematol 115:186-94.nStamm C, Kleine HD, Choi YH, et al. 2003. Autologous bone marrow stem cell transplantation for myocardial regeneration after myocardial infarction. Lancet 361:45-6.nStrauer BE, Brehm M, Zeus T, et al. 2001. Intracoronary, human autologous stem cell transplantation for myocardial regeneration following myocardial infarction [in German]. Dtsch Med Wochenschr 126:932-8.nSutherland DR, Anderson L, Keeney M, Navar R, Chin-Yee I. 1996. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 5:213-26.n

Published

2012-04-26

How to Cite

Ghodsizad, A., Bordel, V., Bruckner, B., Loebe, M., Fuerst, G., Mirsaidi, I., Sucker, M., Ruhparwar, A., Karck, M., & Klein, H. M. (2012). Clinical Labeling and Imaging of Transplanted CD133<sup>+</sup>/CD34<sup>+</sup> Stem Cells in Patients with Ischemic Heart Disease. The Heart Surgery Forum, 15(2), 116-E118. https://doi.org/10.1532/HSF98.20111138

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