The Changes of Gene Expression and Protein Level of Stretch-Activated Chloride Channel in Atrial Myocardium of Dogs with Atrial Fibrillation
Keywords:Atrial fibrillation, Chloride channel, Heart failure, cardiovascular disease
Introduction: ICl,stretch have been reported to be involved in the development of atrial fibrillation, so we observed the changes of transcription and translation levels of ICl,stretch in isolated atrial myocardium of heart failure canine models.
Material and methods: In the control group (N = 10), five dogs were untreated and the other five received sham operation, while dogs in the heart failure group (N = 10) were implanted with cardiac pacemakers and underwent right ventricular pacing to induce heart failure. Cardiac structure and function were evaluated. The gene expression and protein level of ICl,stretch in the left atrial appendage were detected.
Results: The left atrial diameter, right atrial dimension, left ventricular diastolic dimension, and right ventricular diastolic dimension were significantly larger in the heart failure group (P < 0.05). In contrast, the ejection fraction and the left ventricular shorten fraction were higher in the control group (P < 0.05). Both the mRNA and protein expression levels of ICl,stretch in atrial myocardium of the heart failure group were significantly higher compared with the control group.
Conclusion: ICl,stretch might play an important role in the vulnerability to atrial fibrillation in dilated atria with heart failure and could be a potential therapeutic target for atrial fibrillation.
Arnaout MS and Alam SE. 2003. "Congestive heart failure: new paradigms and therapeutic targets." Le Journal médical libanais. The Lebanese medical journal. 51(3): 169.
Baumgarten CM and Clemo HF. 2003. "Swelling-activated chloride channels in cardiac physiology and pathophysiology." Progress in Biophysics & Molecular Biology. 82(1): 25-42.
Bode F, Sachs F, and Franz MR. 2001. "Tarantula peptide inhibits atrial fibrillation." Nature. 409(6816): 35-36.
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, and Jemal A. 2018. "Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries." CA: a cancer journal for clinicians. 68(6): 394-424.
Clemo HF and Baumgarten CM. 1997. "Swelling-activated Gd3+-sensitive cation current and cell volume regulation in rabbit ventricular myocytes." The Journal of General Physiology. 110(3): 297-312.
Clemo HF, Stambler BS, and Baumgarten CM. 1998. "Persistent Activation of a Swelling-Activated Cation Current in Ventricular Myocytes From Dogs With Tachycardia-Induced Congestive Heart Failure." Circulation Research. 83(2): 147-157.
Crozatier B. 1996. "Stretch-induced modifications of myocardial performance: from ventricular function to cellular and molecular mechanisms." Cardiovascular Research. 32(1): 25-37.
Danetz JS, Daviesb RD, Clemo HF, and Baumgarten CM. 2000. "Rabbit ventricular myocyte volume changes as a direct result of crystalloid cardioplegia in congestive heart failure induced by aortic regurgitation." The Journal of Thoracic and Cardiovascular Surgery. 119(4): 826-833.
Duan DD. 2011. "The ClC-3 chloride channels in cardiovascular disease." Acta Pharmacologica Sinica. 32(6): 675-684.
Franz MR and Bode F. 2003. "Mechano-electrical feedback underlying arrhythmias: the atrial fibrillation case." Progress in Biophysics & Molecular Biology. 82: 163-174.
Gupta VM, P. S. P. 2016. "Studies on Chloride Channels and their Modulators." Current Topics in Medicinal Chemistry. 16(16): 1862-1876.
Hagiwara N, Masuda H, Shoda M, and Irisawa H. 1992. "Stretch-activated anion currents of rabbit cardiac myocytes." The Journal of Physiology. 456(1): 285-302.
Inoue R, Jensen LJ, Shi J, Morita H, Nishida M, Honda A, and Ito Y. 2006. "Transient Receptor Potential Channels in Cardiovascular Function and Disease." Circulation Research. 99(2): 119-131.
Jentsch TJ, Friedrich T, Schriever A, and Yamada H. 1999. "THE CLC CHLORIDE CHANNEL FAMILY." Pflügers Archiv: European Journal of Physiology. 437(6): 783-795.
Jentsch TJ and Pusch M. 2018. "CLC Chloride Channels and Transporters: Structure, Function, Physiology, and Disease." Physiological Reviews. 98(3): 1493-1590.
Kamkin A, Kiseleva I, and Isenberg G. 2000. "Stretch-activated currents in ventricular myocytes: amplitude and arrhythmogenic effects increase with hypertrophy." Cardiovascular Research. 48(3): 409-420.
Kamkin A, Kiseleva I, Wagner K, Bohm J, Theres H, Gunther J, and Scholz H. 2003. "Characterization of stretch-activated ion currents in isolated atrial myocytes from human hearts." Pflügers Archiv: European Journal of Physiology. 446(3): 339-346.
Li D, Melnyk P, Feng J, Wang Z, Petrecca K, Shrier A, and Nattel S. 2000. "Effects of Experimental Heart Failure on Atrial Cellular and Ionic Electrophysiology." Circulation. 101(22): 2631-2638.
Lu YY, Cheng CC, Tsai CF, Lin YK, Lee TI, Chen YC, Chen S, and Chen YJ. 2017. "Discrepant effects of heart failure on electrophysiological property in right ventricular outflow tract and left ventricular outflow tract cardiomyocytes." Clinical Science. 131(12): 1317-1327.
Rosamond W, Flegal KM, Furie KL, Go AS, Greenlund KJ, Haase N, Hailpern SM, Ho M, Howard VJ, and Kissela B. 2008. "Heart Disease and Stroke Statistics—2008 Update. A Report From the American Heart Association Statistics Committee and Stroke Statistics Subcommittee." Circulation. 117(4).
Sadoshima J and Izumo S. 1997. "THE CELLULAR AND MOLECULAR RESPONSE OF CARDIAC MYOCYTES TO MECHANICAL STRESS." Annual Review of Physiology. 59(1): 551-571.
Sato R and Koumi S. 1998. "Characterization of the Stretch-activated Chloride Channel in Isolated Human Atrial Myocytes." The Journal of Membrane Biology. 163(1): 67-76.
Seol CA, Kim WT, Ha JM, Choe H, Jang YJ, Youm JB, Earm YE, and Leem CH. 2008. "Stretch-activated currents in cardiomyocytes isolated from rabbit pulmonary veins." Progress in Biophysics & Molecular Biology. 97(2): 217-231.
Slostad BD, Khalsa TK, Young KA, Guerra H, and Bhagra A. 2020. "A case-based approach to integrative medicine for cardiovascular disease prevention." Journal of Integrative Medicine. 18(2): 159-162.
Sridhar A, Nishijima Y, Terentyev D, Khan M, Terentyeva R, Hamlin RL, Nakayama T, Gyorke S, Cardounel AJ, and Carnes CA. 2009. "Chronic heart failure and the substrate for atrial fibrillation." Cardiovascular Research. 84(2): 227-236.
Suchyna TM, Johnson JH, Hamer K, Leykam JF, Gage DA, Clemo HF, Baumgarten CM, and Sachs F. 2000. "Identification of a Peptide Toxin from Grammostola spatulata Spider Venom That Blocks Cation-Selective Stretch-Activated Channels." The Journal of General Physiology. 115(5): 583-598.
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