EFFECT OF ILOPROST, A PROSTACYCLINE ANALOGUE, ON MYOCARDIAL ISCHEMIA-REPERFUSION INJURY

  • Naim Boran Tumer Department of Cardiovascular Surgery, University of Health Sciences Ankara Numune Education and Training Hospital, Ankara, Turkey
  • Gokhan Erol Department of Cardiovascular Surgery, University of Health Sciences Gulhane Education and Training Hospital, Ankara, Turkey
  • Atike Tekeli Kunt Department of Cardiovascular Surgery, University of Health Sciences Ankara Numune Education and Training Hospital, Ankara, Turkey
  • Suat Doganci Department of Cardiovascular Surgery, University of Health Sciences Gulhane Education and Training Hospital, Ankara, Turkey

Abstract

ABSTRACT

 

Myocardial ischemia-reperfusion injury continues to be observed during open heart surgery. Various experimental models have been developed to overcome this injury and to increase postopreative prognosis.

This study was conducted to assess the effect that iloprost, a prostacycline analogue, can have on myocardial ischemia-reperfusion injury.  We evaluated tissue damage by measuring the levels of malonyldialdehyde (MDA), glutathione, and nitric oxide (NO) in tissue and perfusates.

In this study, 20 guinea pig hearts were prepared using the modified Langendorf perfusion apparatus to form control (n=10) and experimental study groups (n=10). Following a pre-ischemic period of perfusion and an ischemic period of 20 minutes, control hearts were perfused with Krebs Hanseleit solution. In the experimental group, iloprost (0.45 mg/kg/hour) was included in the perfusates for the last 10 minutes of the preischemic phase. Following cardiac stabilization, heart rate (pulse/minute), contractility (mm), and aortic pressure (mmHg) values were recorded at the end of pre-ischemia, post-ischemia and reperfusion periods. Perfusate and tissue analyses for glutathione, MDA, and NO levels were made in each group at the end of experiments.

Iloprost was found to have protective effects against myocardial ischemia by means of increased myocardial contractility, decreased tissue/perfusate glutathione levels and inhibited rise of tissue/perfusate MDA observed in the “iloprost-treated” experimental group. Future investigations on myocardial ischemia-reperfusion injury must evaluate iloprost-related mechanisms.

Short Title: Iloprost and ischemia – reperfusion injury

 

Subject Codes : Iloprost, ischemia and reperfusion injury, glutathione, MDA, NO

References

REFERENCES

-Edmunds LH Jr. (2002). The evolution of cardiopulmonary bypass : lessons to be learned. Perfusion 17 (4): 243-51.

-Forman MB, Virmani R, Puett DW. (1990) Mechanisms and therapy of myocardial reperfusion injury. Circulation (81 3 Suppl): IV69-78.

-Gardner TJ, Stewart R, Casale, AS Downey, JM Chambers. (1983)Reduction of myocardial ischemic injury with oxygen-derived free radical scavengers. Surgery 94(3): 423-27.

- Das DK, Engelman RM, Rousou JA, Breyer RH, Otani H, Lemeshow S.(1986). Pathophysiology of superoxide radical; a potential mediator of reperfusion injury in pig hearts. Basic Res Cardiol 81(2): 155-66.

- Simpson PJ, Mickelson J, Fantone JC, Gallagher KP, Lucchesi Br.(1987). Sustained myocardial protection by iloprost with prolonged infusion in a canine model of temporary regional ischaemia. Fed Proc 46: 1144.

-Kurtel H, Granger DN, Tso P, Grisham MB. (1992). Vulnerability of intestinal interstitial fluid to oxidant stress. Am J Phy 263 (4 Pt1); Q 573-8.

-Nakanishi K, Inoue M, Sugawara E, Sano S. (1997). Ischemic and reperfusion injury of cyanotic myocardium in chronic hypoxic rat model: Changes in cyanotic myocardial antioxidant system. J Thorac Cardiovasc Surg 114(6): 1088-96.

-Swedberg K, Hed P, Wadenvik H, Kutti J. (1987). Central hemodynamic and antiplatelet effects of iloprost-a new prostacyclin analogue-in acute myocardial infarction in man. Eur Heart J 8(4): 362-68.

-Sinclair AJ, Barnett AH, Lunec J. (1990). Free radicals and antioxidant systems in health and disease. Br J Hosp Med 43(5): 334-44.

-Blaustein A, Deneke SM, Stolz RI, Baxter D, Healey N, Fanburg BL. (1989). Myocardial glutathione depletion impairs recovery after short periods of ischemia. Circulation 80(5): 1449-57.

-Ma XL, Weyrich AS, Lefer DJ, Lefer AM. (1993). Diminshed basal nitric oxide release after myocardial ischemia and reperfusion promotes neutrophil adherence to coronary endothelium. Circ Research 72(2): 403-12.

- Sheikh Arshad Saeed, Muhammad Anwar Waqar, Akbar Jaleel Zubairi, Hadi Bhurgri, Abdullah Khan, Saqib Ali Sikander Gowani, Saima N. Waqar, M. Iqbal Choudhary, Saima Jalil, Ali Hyder Zaidi, Iffat Ara.(2005). Myocardial ischaemia and reperfusion injury : Reactive oxygen species and the role of neutrophil. JCPSP 15(8 ): 507-514.

- Jeremy D. Flynn, Wendell S. Akers . (2003) Effects of the Angiotensin II Subtype 1 Receptor Antagonist Losartan on Functional Recovery of Isolated Rat Hearts Undergoing Global Myocardial Ischemia-Reperfusion. Pharmacotherapy 23 (11) :1401-1410.

-Turan Belma, Harjot K. Saini, Ming Zhang, Dashang Prajapati, Vijayan Elimban, Naranjan S. Dhalla. (2005). Selenium Improves Cardiac Function by Attenuating the Activation of NF- B Due to Ischemia Reperfusion Injury. Antioxid. Redox Signal 7 (9-10 ): 1388-1397.

- S Pucheu, C Coudray, N Tresallet, A Favier J de Leiris. (1993). Effect of iron overload in the isolated ischemic and reperfused rat heart. Cardiovascular Drugs and Therapy (Historical Archive)( 7 ) ; 4, 701-711.

- Ding YF, Li YL, Ho SY.(1996). Ischemic preconditioning and exogenous L-arginine reduce infarct size in rabbit heart ( Abstract ). Sheng Li Xue Bao 48 ( 6 ):564-70.

- Ambrosio G, Villari B, Chiariello M. (1992). Calcium antagonists and experimental myocardial ischemia reperfusion injury. J Cardiovasc Pharmacol.;20 Suppl 7: S26-9.

-Y. Qiu, M. Galinanes, D. J. Hearse. (1995). Protective effect of Nicorandil as an additive to the solution for continous warm cardioplegia. J. Thorac. Cardiovasc. Surg. (October 1) 110(4): 1063 - 1072.

Published
2019-01-30
Section
Articles