Effects of Cilostazol and Diltiazem Hydrochloride on Ischemia-Reperfusion Injury in a Rat Hindlimb Model

Bekir Ä°NAN; Selma SÖNMEZ ERGÜN; Asiye NURTEN; Canan KÜÇÜKGERGİN; Aslı ZENGİN TÜRKMEN; Şule SEÇKİN; Kerem ERKALP; Sedat ZÄ°YADE

doi:10.1532/hsf.1663

Authors

Bekir Ä°NAN Department of Cardiothoracic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul
Selma SÖNMEZ ERGÜN Department of Plastic, Reconstructive and Aesthetic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul,
Asiye NURTEN Department of Physiology, Faculty of Medicine, Yeni Yuzyil University, Istanbul,
Canan KÜÇÜKGERGİN Department of Biochemistry, Istanbul Medical School, Istanbul University, Istanbul,
Aslı ZENGİN TÜRKMEN Department of Physiology, Faculty of Medicine, Yeni Yuzyil University, Istanbul,
Şule SEÇKİN Department of Biochemistry, Istanbul Medical School, Istanbul University, Istanbul,
Kerem ERKALP Department of Anesthesiology and Reanimation, Bağcılar Research and Educational Hospital, Istanbul,
Sedat ZÄ°YADE Department of Thoracic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul,

DOI:

https://doi.org/10.1532/hsf.1663

Abstract

Objective: Free radicals and neutrophils are potent sources of ischemia-reperfusion injury (I/R) and they can be limited by the use of exogenous application of some therapeutic agents. The objective of this study was to compare the effects of cilostazol and diltiazem hydrochloride in a rat hind limb model of I/R injury.

Methods: Skeletal muscles submitted to 2 hours of ischemia by placing an aneurysm clip to femoral artery and reperfused after 1, 2 and 4 hours. Seventy-two Wistar-Albino rats were randomly divided into mainly four groups according to treatment agents: Group I (control group) was treated with saline; Group II was treated with diltiazem hydrochloride; Group III was treated with cilostazol in 30% dimethyl sulphoxide; and Group IV was treated with 30% dimethyl sulphoxide intraperitoneally. These four main groups also subdivided into three subgroups according to duration of the reperfusion times. Blood samples were taken and all rats were sacrificed.

Results: Cilostazol-treated groups demonstrated a significant decrease in tissue and serum malondialdehyde (MDA) levels, and tissue myeloperoxidase (MPO ) activity compared with other groups. Increase in serum nitric oxide (NOx) level was significantly higher in all subgroups of cilastazol, diltiazem hydrochloride, and dimethyl sulphoxide groups versus the control group.

Conclusion: Although these results suggest the beneficial effects of cilostazol and diltiazem hydrochloride on I/R injury, the effect of cilostazol on I/R injury seems to be more efficient than diltiazem hydrochloride.

Author Biographies

Bekir Ä°NAN, Department of Cardiothoracic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul

Department of Cardiothoracic Surgery, Bezmialem Medical School,

Selma SÖNMEZ ERGÜN, Department of Plastic, Reconstructive and Aesthetic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul,

Deparment of Plastic Reconstructive and Aesthetic Surgery, Bezmialem Medical School

Asiye NURTEN, Department of Physiology, Faculty of Medicine, Yeni Yuzyil University, Istanbul,

Department of Physiology, Faculty of Medicine,

Canan KÜÇÜKGERGİN, Department of Biochemistry, Istanbul Medical School, Istanbul University, Istanbul,

Department of Biochemistry, Istanbul Medical School,

Aslı ZENGİN TÜRKMEN, Department of Physiology, Faculty of Medicine, Yeni Yuzyil University, Istanbul,

Department of Physiology, Faculty of Medicine,

Şule SEÇKİN, Department of Biochemistry, Istanbul Medical School, Istanbul University, Istanbul,

Department of Biochemistry, Istanbul Medical School,

Kerem ERKALP, Department of Anesthesiology and Reanimation, Bağcılar Research and Educational Hospital, Istanbul,

Department of Anesthesiology and Reanimation,

Sedat ZÄ°YADE, Department of Thoracic Surgery, Bezmialem Medical School, Bezmialem Vakif University, Istanbul,

Department of Thoracic Surgery, Bezmialem Medical School,

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