@article{Haapanen_Herajärvi_Honkanen_Mustonen_Tuominen_Puistola_Anttila_Juvonen_2018, title={Immunohistochemical Analysis of the Spinal Cord Ischemia– Effect of Remote Ischemic Preconditioning in a Porcine Model}, volume={21}, url={https://journal.hsforum.com/index.php/HSF/article/view/1969}, DOI={10.1532/hsf.1969}, abstractNote={<p class="p1"><span class="s1"><strong>Background:</strong> In experimental settings, remote ischemic preconditioning (RIPC) has shown a positive effect regarding spinal cord protection after local ischemia. In this study, we conducted spinal cord immunohistochemistry to demonstrate the protective effect of RIPC after 24 hours of the regional ischemia.<span class="Apple-converted-space"> </span></span></p><p class="p1"><span class="s1"><strong>Methods:</strong> Twenty piglets were randomized into an RIPC group (n = 10) and a control group (n = 10). The RIPC group underwent transient left hind limb ischemia before systematic left subclavian artery and segmental artery occlusion at the level of the diaphragm. Twenty-four hours later, the thoracic and lumbar spinal cords were harvested, and the oxidative stress markers were immunohistochemically analysed.<span class="Apple-converted-space"> </span></span></p><p class="p1"><span class="s1"><strong>Results:</strong> A total of 18 animals survived the 4-hour follow up (10 in the RIPC group, 8 in the control group) and 14 animals survived the 24-hour follow up (7 in each group). In the single sections of the spinal cord, the antioxidant pathway activation was seen in the RIPC group, as OGG1 and DJ-1/PARK7 activation was higher (<em>P</em> = .038 and <br /> <em>P</em> = .047, respectively).<span class="Apple-converted-space"> </span></span></p><p class="p1"><span class="s1"><strong>Conclusions:</strong> The results indicate that the neuroprotective effect of RIPC on the spinal cord after local ischemic insult remains controversial.</span></p>}, number={3}, journal={The Heart Surgery Forum}, author={Haapanen, Henri Johannes and Herajärvi, Johanna and Honkanen, Hannu-Pekka and Mustonen, Caius and Tuominen, Hannu and Puistola, Ulla and Anttila, Vesa and Juvonen, Tatu}, year={2018}, month={May}, pages={E209-E214} }