Do N-Acetylcystein, ß-Glucan, and Coenzyme Q10 Mollify Myocardial Ischemia-Reperfusion Injury?

Authors

  • Cengiz Bolcal
  • Vedat Yildirim
  • Suat Doganci
  • Murat Sargin
  • Ahmet Aydin
  • Erkan Kuralay
  • Ertugrul Ozal
  • Ufuk Demirkilic
  • Bilgehan Savas Oz
  • Ahmet Sayal
  • Harun Tatar

DOI:

https://doi.org/10.1532/HSF98.20061195

Abstract

Background. N-acetylcysteine, ?-glucan, and coenzyme Q10 have been shown to have antioxidant and anti-inflammatory effects on reperfusion injury. The aim of our study was to determine and evaluate the effects of these agents on myocardial ischemia-reperfusion injury.

Methods. Forty-four New Zealand white rabbits, all female, weighing 2.4 to 4.1 kg (mean, 3.6 kg) were used in the study. Four study groups of 11 animals were arranged by randomization. The groups were the control group (group C), a group premedicated with coenzyme Q10 (group Q), a group premedicated with ?-glucan (group ?T), and a group premedicated with N-acetylcysteine (group N). After exploration of the heart, a basal myocardial biopsy was taken from the anteroapical left ventricle, and the first blood sampling was done before ischemia. For the ischemia-reperfusion experiments, the major left anterior descending artery was occluded after baseline measurements. After a 45-minute transient ischemic period, the heart was perfused for 120 minutes. After perfusion, the second myocardial biopsy was taken from the anteroapical left ventricle, and the second blood sampling was done. Blood and tissue analysis were performed and evaluated statistically.

Results. Baseline and reperfusion levels of glutathione peroxidase, superoxide dismutase, malonyldialdehyde, and nitric oxide changed significantly. While malonyldialdehyde levels increased in group C, they decreased in the other study groups (P =.001). The increases in glutathione peroxidase and superoxide dismutase levels were significant in all groups except group C (P =.0001 and P <.05, respectively). Levels of nitric oxide were found to be decreased in group C, whereas they increased in the other groups (P =.001).

Conclusion. Antioxidant medication may help in lowering the risk of myocardial ischemia-reperfusion injury. All the medications in our study are shown to have effective roles in preventing ischemia-reperfusion injury to some extent through their antioxidant properties.

References

Andersen LW, Thiis J, Kharazmi A, Rygg I. 1995. The role of N-acetylcysteine administration on the oxidative response of neutrophils during cardiopulmonary bypass. Perfusion 10:21-6.nAydin A, Orhan H, Sayal A, Ozata M, Sahin G, Isimer A. 2001. Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: effects of glycemic control. Clin Biochem 34:65-70.nCeconi C, Curello S, Cargnoni A, Ferrari R, Albertini A, Visioli O. 1988. The role of glutathione status in the protection against ischaemic and reperfusion damage: effects of N-acetylcysteine. J Mol Cell Cardiol 20:5-13.nChello M, Mastroroberto P, Romano R, et al. 1994. Protection by coenzyme Q10 from myocardial reperfusion injury during coronary artery bypass grafting. Ann Thorac Surg 58:1427-32.nChew GT, Watts GF. 2004. Coenzyme Q10 and diabetic endotheliopathy: oxidative stress and the ‘recoupling hypothesis.’ Q J Med 97:537-48.nCotgreave IA. 1997. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. In: Antioxidants in Disease Mechanisms and Therapy. Dies H, ed. Academic Press; San Diego, CA: 205-21.nCuzzocrea S, Rossi A, Serraino I, et al. 2004. Role of 5-lipoxygenase in the multiple organ failure induced by zymosan. Int Care Med 30:1935-43.nFitzgerald SP, Campbell JJ, Lamont JV. 1992. The establishment of reference renges for selenium. Selenoenzme glutathione peroxidase and the metalloenzyme superoxide dismutase in blood fractions. The fifth international symposium on selenium in biology and medicine, Tennessee, July 20-23, 1992.nJennings RB, Sommers HM, Smyth GA, Flack HA, Linn H. 1960. Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog. Pathology 70:68-78.nKayali H, Ozdag MF, Kahraman S, et al. 2005. The antioxidant effect of beta-glucan on oxidative stress status in experimental spinal cord injury in rats. Neurosurg Rev 28:298-302.nMarchioli R. 1999. Antioxidant vitamins and prevention of cardiovascular disease: laboratory, epidemiological and clinical trial data. Pharmacol Res 40:227-38.nMenasche P, Grousset C, Gauduel Y, Mouas C, Piwnica A. 1992. Maintenance of the myocardial thiol pool by N-acetylcysteine: an effective means of improving cardioplegic protection. J Thorac Cardiovasc Surg 103:936-44.nMortensen SA. 1996. Coenzyme Q10 treatment may be protective during coronary artery bypass operations. Ann Thorac Surg 62:1243-4.nNaranjan SD, Adel BE, Tomoji H, Naoki M. 2000. Status of myocardial antioxidants in ischemia-reperfusion injury. Cardiovasc Res 47:446-56.nOhkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animals and tissues by thiobarbituric acid reaction. Anal Biochem 95:351-8.nOrhan G, Yapici N, Yuksel M, et al. 2006. Effects of N-acetylcysteine on myocardial ischemia reperfusion injury in bypass surgery. Heart Vessels 21:42-7.nPark JL, Lucchesi BR. 1999. Mechanisms of myocardial reperfusion injury. Ann Thorac 68:1905-12.nPleban PA, Munyani A, Bechum J. 1982. Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes. Clin Chem 2:311-6.nRosenfeldt F, Marasco S, Lyon W, et al. 2005. Coenzyme Q10 therapy before cardiac surgery improves mitochondrial function and in vitro contractility of myocardial tissue. J Thorac Cardiovasc Surg 129:25-32.nSchaper W, Schaper J. 1997. Reperfusion injury: an opinionated view. J Thrombos Thrombolysis 4:113-6.nSochman J. 2002. N-acetylcysteine in acute cardiology: 10 years later. What do we know and what would we like to know. JACC 9:1422-8.nSunamori M, Tanaka H, Maruyama T, Sultan I, Sakamoto T, Suzuki A. 1991. Clinical experience of coenzyme Q10 to enhance intraoperative myocardial protection in coronary artery revascularization. Cardiovasc Drugs Ther 5(suppl 2):297-300.nTang LD, Sun JZ, Wu K, Sun CP, Tang ZM. 1991. Beneficial effects of N-acetylcysteine and cysteine in stunned myocardium in perfused rat heart. Br J Pharmacol 102:601-6.nTracey WR, Tse J, Carter G. 1995. Lipopolisaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther 272:1011-5.n

Published

2007-04-24

How to Cite

Bolcal, C., Yildirim, V., Doganci, S., Sargin, M., Aydin, A., Kuralay, E., Ozal, E., Demirkilic, U., Oz, B. S., Sayal, A., & Tatar, H. (2007). Do N-Acetylcystein, ß-Glucan, and Coenzyme Q10 Mollify Myocardial Ischemia-Reperfusion Injury?. The Heart Surgery Forum, 10(3), E222-E227. https://doi.org/10.1532/HSF98.20061195

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