Association between Circulatory and Plaque Resistin Levels with Carotid Plaque Instability and Ischemic Stroke Events

  • Ivana Jurin Department of Cardiovascular Diseases, University Hospital Dubrava, Avenija Gojka Šuška 6, 10 000 Zagreb, Croatia
  • Frane Paić Laboratory for Epigenetics and Molecular medicine, Department of Biology and Medical Genetics, School of Medicine, University of Zagreb, Šalata 3, Zagreb, Croatia
  • Stela Bulimbašić Department of Pathology and Cytology, University Hospital Centre Zagreb, Avenija Gojka Šuška 6, 10 000 Zagreb, Croatia
  • Igor Rudež Department of Cardiac and Transplantation Surgery, University Hospital Dubrava, Avenija Gojka Šuška 6, 10 000 Zagreb, Croatia
  • Lovorka Đerek Clinical Institute for Chemistry, University Hospital Centre “Sestre Milosrdnice”, Vinogradska cesta 29, 10000 Zagreb, Croatia
  • Hrvoje Jurin Department of Cardiovascular Diseases, University Hospital Centre Zagreb, Kišpatićeva 12, 10000 Zagreb, Croatia
  • Aleksandar Knežević Department of Cardiology, General Hospital Zadar, Bože Peričića 5, 23000 Zadar, Croatia
  • Boris Starcevic Department of Cardiovascular Diseases, University Hospital Dubrava, Avenija Gojka Šuška 6, 10 000 Zagreb, Croatia
  • Marko Ajduk Department of Vascular Surgery, University Hospital Dubrava, Avenija Gojka Šuška 6, 10 000 Zagreb, Croatia

Abstract

ses of ischemic stroke. The risk of ischemic stroke increases with the degree of carotid stenosis and plaque vulnerability. The aim of this study was to investigate the association of circulating and plaque resistin levels with plaque vulnerability and ischemic stroke events in patients with moderate- to high-grade carotid artery stenosis.

Methods: 40 patients with ischemic stroke events and
38 neurologically asymptomatic patients scheduled for carotid endarterectomy were recruited for this study. Fasting blood samples for laboratory analysis were collected preoperatively and serum resistin levels were measured by enzyme-linked immunosorbent assays. Carotid endarterectomy specimens were analyzed according to the gold-standard procedure of histological classification. Plaque resistin expression was determined by standard immunohistochemical procedure.

Results: Serum resistin levels and resistin plaque expression were found to be significantly higher in subjects with unstable carotid plaque (P < .001) while significantly higher serum resistin levels were also present in patients with ischemic stroke events (P < .001). In univariate stepwise logistic regression analysis, higher serum resistin levels were significantly associated with plaque instability (OR 2.223, 95% CI 1.488-3.320, P < .0001) and ischemic stroke events (OR 1.237, 95% CI 1.079-1.420, P = .002). There was also a significant association between higher serum and plaque resistin expression (OR 1.663, 95% CI1.332-2.077, P < .0001). These associations remained significant in all models of multivariate logistic regression analysis. High serum and plaque resistin levels were also significantly associated with specific histological features of plaque instability.

Conclusion: The results suggests that serum resistin levels may be used as a potential biomarker of plaque vulnerability and ischemic stroke events in patients with moderate- to high-grade carotid artery stenosis and highlight the possible relationship that plaque resistin expression has with histological features of plaque vulnerability.

Author Biography

Frane Paić, Laboratory for Epigenetics and Molecular medicine, Department of Biology and Medical Genetics, School of Medicine, University of Zagreb, Šalata 3, Zagreb, Croatia

Department of Biology and Medical Genetics, School of Medicine, University of Zagreb, Šalata 3, Zagreb, Croatia, PhD, Assistant professor

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Published
2018-11-13
Section
Articles